A Pilot Study of Detecting RS920778 and RS1899663 Genetic Variants of HOTAIR in A Cohort of Sporadic Breast Cancer Patients in Sri Lanka Genetic variants of HOTAIR

Sumadee De Silva (1), Asanka Sudeshini Hewage (2), Fathmath Anaan Shafeeu (3), Vahinipriya Manoharan (4), Kanishka De Silva (5)
(1) Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Sri Lanka,
(2) Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Sri Lanka, Sri Lanka,
(3) Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Sri Lanka, Sri Lanka,
(4) Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Sri Lanka, Sri Lanka,
(5) National Cancer Institute, Maharagama, Sri Lanka, Sri Lanka

Abstract

Background: Some functional single nucleotide polymorphisms (SNPs) on the HOTAIR gene are potent to elevate breast cancer risk where rs920778 and rs1899663 are associated with the expression and function of the HOTAIR gene, and these SNPs were detected in the current study.


Methods: A cohort of nine sporadic cancer patients and five healthy controls were screened for selected HOTAIR variants using direct sequencing.


Results: Among patients, seven were with TT genotype, and other 2 with CT genotype for rs920778 C>T while two healthy controls showed TT, another two were with CT, and the remaining with CC genotypes. Among the cohort, the rs1899663 variant was not found in any patients and healthy controls.,


Conclusion:  In the study, the T allele of rs920778 was prominent among the sporadic breast cancer in the studied cohort while only a wild allele (T) of rs1899663 was more prominent in the patients and healthy controls.

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References

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Authors

Sumadee De Silva
sum@ibmbb.cmb.ac.lk (Primary Contact)
Asanka Sudeshini Hewage
Fathmath Anaan Shafeeu
Vahinipriya Manoharan
Kanishka De Silva
1.
De Silva S, Hewage AS, Shafeeu FA, Manoharan V, De Silva K. A Pilot Study of Detecting RS920778 and RS1899663 Genetic Variants of HOTAIR in A Cohort of Sporadic Breast Cancer Patients in Sri Lanka: Genetic variants of HOTAIR. Arch Breast Cancer [Internet]. 2024 May 1 [cited 2024 May 18];11(2):204-7. Available from: https://archbreastcancer.com/index.php/abc/article/view/877

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