Synthesis, Characterization and Evaluation of Antioxidant, Anticancer and Toxicity Properties of Silver Nanoparticles Synthesized from Syzygium Aromaticum SNP synthesized from Syzygium aromaticum

Jambulingam Vishal (1), Soundharajan Ranjani (2), Ravindranath Jenin Karunya (3), Srinivasan Hemalatha (4)
(1) School of Life Sciences, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai, Tamilnadu, India, India,
(2) School of Life Sciences, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai, Tamilnadu, India, India,
(3) School of Life Sciences, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai, Tamilnadu, India, India,
(4) School of Life Sciences, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai, Tamilnadu, India, India

Abstract

Background: Syzygium aromaticum, also known as clove, and its essential oil has already been proved to have antioxidant, anti-inflammatory and anticancer properties. Clove is used in various foods owing to its potent antimicrobial and antioxidant properties. Essential oil extracted from clove has been used in traditional medicine for treating various ailments.


Methods: In silico analyses of phytocompounds of Syzygium aromaticum namely eugenol, B-caryophyllene, gallic acid, crategolic acid, kaempferol, quercetin, cinnamaldehyde, and oleanolic acid were docked with three apoptotic proteins involved in breast cancer, namely BCL-2, BAX and APAF-1 using AUTODOCK. In addition, flower bud extract of Syzygium aromaticum was used for the synthesis of AgNPs (silver nanoparticles). The synthesized clove-silver nanoparticles were then characterized using various techniques such as Ultraviolet-visible spectrophotometry, FTIR, FESEM-EDX, DLS and zeta potential to determine the particle size, shape, crystalline structure, and stability of CL-AgNPs and tested for its anticancer potential in MCF-7 cell lines. 


Results: In silico analysis predicted that phytochemicals of clove have good interactions with the apoptosis related proteins of breast cancer. In vitro assay confirmed the cytotoxic effect of the synthesized CL-AgNPs on breast cancer cells using the MCF-7 cell line with the IC50 value of 58.64 µg/ml.


Conclusion: In vitro analysis of the anticancer activity of CL-AgNPs in MCF-7 cell line supports the in silico study by proving active interactions between the phytochemicals of clove and target proteins of the breast cancer and hence Syzygium aromaticum has been proved to possess potential anticancer property. Further research is needed to consider clove-silver nanoparticles as a novel drug for treating breast cancer.

Full text article

Generated from XML file

References

Sun YS, Zhao Z, Yang ZN, Xu F, Lu HJ, Zhu ZY, et al. Risk Factors and Preventions of Breast Cancer. International Journal of Biological Sciences 2017;13(11):1387-1397. doi:10.7150/ijbs.21635.

Buttacavoli M, Albanese N, Cara G, Alduina R, Faleri C, Gallo M, et al. Anticancer activity of biogenerated silver nanoparticles: an integrated proteomic investigation. Oncotarget. 2017;9(11):9685-9705. doi: 10.18632/oncotarget.23859.

Alvandi N, Rajabnejad M, Taghvaei Z, Esfandiari N. New generation of viral nanoparticles for targeted drug delivery in cancer therapy. Journal of Drug Targeting. 2022;30(2):151-165. doi: 10.1080/1061186X.2021.1949600.

Esfandiari N, Arzanani MK, Koohi-Habibi M. The study of toxicity and pathogenicity risk of Potato Virus X/Herceptin nanoparticles as agents for cancer therapy. Cancer Nanotechnology. 2018; 9:1. doi: 10.1186/s12645-018-0036-6.

Madhumitha H, Ranjani S, Hemalatha S. Clitoria ternatea floral mediated Synthesis, characterization, antioxidant and cytotoxicity evaluation of silver nanoparticles. Archives of Breast Cancer. 2023;10(3): In-Press.

Esfandiari N. Targeting Breast Cancer With Bio-inspired Virus Nanoparticles. Archives of Breast Cancer. 2018; 5(2):90-5. doi: 10.19187/abc.20185290-95.

Taherian A, Esfandiari N. Nanomedicine, A New Therapeutic Strategy in Breast Cancer treatment. Archives of Breast Cancer. 2019;6(2):67-78. doi: 10.32768/abc.20196267-78.

Jamshidi-Kia F, Lorigooini Z, Amini-Khoei H. Medicinal plants: past history and future perspective. Journal of Herbmed Pharmacology. 2018;7(1):1-7. doi: 10.15171/jhp.2018.01.

Batiha GE, Alkazmi LM, Wasef LG, Beshbishy AM, Nadwa EH, Rashwan EK. Syzygium aromaticum L. (Myrtaceae): Traditional Uses, Bioactive Chemical Constituents, Pharmacological and Toxicological Activities. Biomolecules. 2020; 10(2):202. doi: 10.3390/biom10020202.

Han X, Parker TL. Anti-inflammatory activity of clove (Eugenia caryophyllata) essential oil in human dermal fibroblasts. Pharmaceutical biology. 2017;55(1):1619-1622. doi: 10.1080/13880209.2017.1314513.

Pinzi L, Rastelli G. Molecular Docking: Shifting Paradigms in Drug Discovery. International Journal of Molecular Sciences. 2019;20(18):4331. doi: 10.3390/ijms20184331.

Radha G, Raghavan SC. BCL2: A promising cancer therapeutic target. Biochimica et Biophysica Acta - Reviews on Cancer. 2017;1868(1):309-314. doi: 10.1016/j.bbcan.2017.06.004.

Ahmed RH, Mustafa DE. Green synthesis of silver nanoparticles mediated by traditionally used medicinal plants in Sudan. International Nano Letters. 2020;10(1):1–14. doi: 10.1007/s40089-019-00291-9.

Jalalvand A, Khatouni S, Najafi Z, Fatahinia F, Ismailzadeh N, Farahmand B. Computational drug repurposing study of antiviral drugs against main protease, RNA polymerase, and spike proteins of SARS-CoV-2 using molecular docking method. Journal of Basic and Clinical Physiology and Pharmacology. 2021;33(1):85-95. doi: 10.1515/jbcpp-2020-0369

Rangsinth P, Sillapachaiyaporn C, Nilkhet S, Tencomnao T, Ung AT, Chuchawankul S. Mushroom-derived bioactive compounds potentially serve as the inhibitors of SARS-CoV-2 main protease: An in silico approach. Journal of Traditional and Complementary Medicine. 2021;11(2):158-172. doi: 10.1016/j.jtcme.2020.12.002.

Daina A, Michielin O, Zoete V. SwissADME: a free web tool to evaluate pharmacokinetics, drug-likeness and medicinal chemistry friendliness of small molecules. Scientific Reports. 2017;7:42717. doi: 10.1038/srep42717.

Lipinski CA, Lombardo F, Dominy BW, Feeney PJ. Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings. Advanced Drug Delivery Reviews. 2001;46(1-3):3-26. doi: 10.1016/s0169-409x(00)00129-0.

Archna A, Halima R. A review on green synthesis of silver nanoparticle, characterization and optimization parameters. International Journal of Research in Engineering and Technology. 2016; 5(15);49-53. doi: 10.15623/ijret.2016.0527010.

Kumar AS, Madhu G, John E, Kuttinarayanan SV, Nair SK. Optical and antimicrobial properties of silver nanoparticles synthesized via green route using honey. Green Processing and Synthesis. 2020;9(1):268-274. doi: 10.1515/gps-2020-0029.

Sai Nivetha S, Ranjani S, Hemalatha S. Synthesis and application of silver nanoparticles using Cissus quadrangularis. Inorganic and Nano Metal Chemistry. 2020. doi: 10.1080/24701556.2020.1862219.

Lawrence AA, Prakash JTJ. Biogenic synthesis of silver nanoparticles using manilkarahexandra (roxb.) dubard stem bark extract and it’s physical, chemical characterization and pharmaceutical evaluation. International Journal of Applied Pharmaceutics. 2019;11(3):79–88. doi: 10.22159/ijap.2019v11i3.31403.

Arulvasu C, Jennifer SM, Prabhu D, Chandhirasekar D. Toxicity Effect of Silver Nanoparticles in Brine Shrimp Artemia., The Scientific World Journal. 2014:256919. doi: 10.1155/2014/256919.

Ravindranath KJ, Christian SD, Srinivasan H. Screening of Anti-carcinogenic Properties of Phytocompounds from Allium ascalonicum for Treating Breast Cancer Through In Silico and In Vitro Approaches. Applied Biochemistry and Biotechnology. 2023;195(2):1136-1157. doi: 10.1007/s12010-022-04202-1.

Jain R, Grover A. Maslinic acid differentially exploits the MAPK pathway in estrogen-positive and triple-negative breast cancer to induce mitochondrion-mediated, caspase-independent apoptosis. Apoptosis. 2020;25(11-12):817-834. doi: 10.1007/s10495-020-01636-y.

Kim SH, Hwang KA, Choi KC. Treatment with kaempferol suppresses breast cancer cell growth caused by estrogen and triclosan in cellular and xenograft breast cancer models. The Journal of Nutrional Biochemistry. 2016;28:70-82. doi: 10.1016/j.jnutbio.2015.09.027.

Wang X, Yang Y, An Y, Fang G. The mechanism of anticancer action and potential clinical use of kaempferol in the treatment of breast cancer. Biomedicine Pharmacotherapy. 2019;117:109086. doi: 10.1016/j.biopha.2019.109086.

Ezzati M, Yousefi B, Velaei K, Safa A. A review on anti-cancer properties of Quercetin in breast cancer. Life Science. 2020;248:117463. doi: 10.1016/j.lfs.2020.117463.

Javan Bakht Dalir S, Djahaniani, H, Nabati F, Hekmati M. Characterization and the evaluation of antimicrobial activities of silver nanoparticles biosynthesized from Carya illinoinensis flower extract. Heliyon. 2020;6(3):E03624. doi: 10.1016/j.heliyon.2020.e03624.

Deepashree CL, Kumar KKJ, Prasad AGD, Zarei M, Gopal S. FTIR spectroscopic studies on cleome gynandra – comparative analysis of functional group before and after extraction. Romanian Journal of Biophysics. 2013;22(3-4):137-143. Available from: https://www.rjb.ro/articles/353/art01Shree.pdf

Ramola B, Joshi NC. Green Synthesis, Characterisations and Antimicrobial Activities of CaO Nanoparticles. Oriental Journal of Chemistry. 2019;35(3):1154-1157. doi: 10.13005/ojc/350333.

Roy P, Das B, Mohanty A, Mohapatra S. Green synthesis of silver nanoparticles using Azadirachta indica leaf extract and its antimicrobial study. Applied Nanoscience. 2017;7:843–850. doi: 10.1007/s13204-017-0621-8.

Lakshya M, Ranjani S, Shariq Ahmed M, Jeya Shree T, Akther T, Poompavai S et al. Turmeric-silver-nanoparticles for effective treatment of breast cancer and to break CTX-M-15 mediated antibiotic resistance in Escherichia coli. Inorganic and Nano Metal Chemistry. 2021;51(6). doi: 10.1080/24701556.2020.1812644.

Li Z, Ye L, Liu J, Lian D, Li X. Sorafenib-Loaded Nanoparticles Based on Biodegradable Dendritic Polymers for Enhanced Therapy of Hepatocellular Carcinoma. Int J Nanomedicine. 2020;15:1469-1480. doi: 10.2147/IJN.S237335.

Mourdikoudis S, Pallares RM, Thanh NTK. Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties. Nanoscale. 2018;10:12871-12934. doi: 10.1039/C8NR02278J

Jayappa MD, Ramaiah CK, Kumar MAP, Suresh D, Prabhu A, Devasya RP et al. Green synthesis of zinc oxide nanoparticles from the leaf, stem and in vitro grown callus of Mussaendafrondosa L.: characterization and their applications. Applied Nanoscience. 2020;10:3057–3074. doi: 10.1007/s13204-020-01382-2.

Yazhiniprabha M, Vaseeharan B. In vitro and in vivo toxicity assessment of selenium nanoparticles with significant larvicidal and bacteriostatic properties. Materials Science and Engineering: C. 2019;103:109763. doi: 10.1016/j.msec.2019.109763.

Authors

Jambulingam Vishal
Soundharajan Ranjani
Ravindranath Jenin Karunya
Srinivasan Hemalatha
hemalatha.sls@bsauniv.ac.in (Primary Contact)
Author Biography

Jambulingam Vishal, School of Life Sciences, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai, Tamilnadu, India

GST road, Vandalur Chennai

1.
Vishal J, Ranjani S, Karunya RJ, Hemalatha S. Synthesis, Characterization and Evaluation of Antioxidant, Anticancer and Toxicity Properties of Silver Nanoparticles Synthesized from Syzygium Aromaticum: SNP synthesized from Syzygium aromaticum. Arch Breast Cancer [Internet]. 2023 Jul. 5 [cited 2024 May 23];10(3):291-300. Available from: https://archbreastcancer.com/index.php/abc/article/view/717

Article Details

Most read articles by the same author(s)