Comparative Analyses of Villin and HER-2 Genes Expression in Breast Cancer

Hadiseh Mohammadpour (1), Mehrdad Hashemi (2), Reza shirkoohi (3), Mojtaba Saffari (4), Amir Nader Emami Razavi (5)
(1) Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran,
(2) Modern Science Department, Tehran Azad University of Medical Sciences, Tehran, Iran,
(3) Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran,
(4) Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran,
(5) Iran National Tumor Bank, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Background: It has been previously demonstrated that HER-2 (human epidermal growth Factor receptor 2) positive breast cancers are associated with an aggressive nature. Villin is an actin bundling protein that plays a key role in actin reorganization and cell remodeling during stress. In this study, we aimed to investigate the correlation of Villin gene expression with HER-2 in breast cancer patients.

Methods: Samples of 42 patients with breast cancer, and 3 controls were collected. Expression of Villin and HER-2 genes were monitored with real-time PCR using pre-designed primers. Student T-test was used to compare the means between the groups.

Results: The mean age of the patients was 50±4.11years. Expression of the Villin gene was decreased in 28 samples (18 and 10 samples with negative and strongly HER-2 positive, respectively). Villin gene expression was increased in 14 samples (7, 2 and 5 samples with negative, weakly positive, and strongly HER-2 positive, respectively). The expression of Villin was significantly correlated with HER-2 positive status (P = 0.00057)

Conclusions: We found that Villin gene expression is associated with HER-2 positivity and may be a predicting factor in aggressive breast cancer.

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Authors

Hadiseh Mohammadpour
Mehrdad Hashemi
Reza shirkoohi
shirkoohi@tums.ac.ir (Primary Contact)
Mojtaba Saffari
Amir Nader Emami Razavi
1.
Mohammadpour H, Hashemi M, shirkoohi R, Saffari M, Emami Razavi AN. Comparative Analyses of Villin and HER-2 Genes Expression in Breast Cancer. Arch Breast Cancer [Internet]. 2015 Nov. 30 [cited 2024 Jun. 15];2(4):120-4. Available from: https://archbreastcancer.com/index.php/abc/article/view/59

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